pathscan rp phospho vegfr 2 tyr1175 sandwich elisa kit  (Cell Signaling Technology Inc)

Journal: Molecules

Article Title: Discovery of a Novel Coumarin/Thiazole Chalcone Hybrid as a Potent Dual Inhibitor of Tubulin and Carbonic Anhydrases IX & XII with Promising Anti-Proliferative Activity

doi: 10.3390/molecules31060917

Figure Lengend Snippet: Effect of compound 6 on VEGF-stimulated VEGFR-2 (Tyr1175) phosphorylation in HUVEC cells.

Article Snippet: Phosphorylated VEGFR-2 (Tyr1175) levels were quantified using the PathScan ® RP Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit (#7335, Cell Signaling Technology, Danvers, MA, USA) following the rapid protocol.

Techniques: Phospho-proteomics

Journal: The Journal of Physiological Sciences : JPS

Article Title: Identification of a small molecule that targets activator of G-protein signaling 8

doi: 10.1016/j.jphyss.2026.100064

Figure Lengend Snippet: Effects of AGS8 inhibitor on interaction assay and VEGF signaling in cells. A, Example of a GST pulldown assay of GST-AGS8 with Gβ 1 γ 2 . GST-fusion protein (10 pmol) and human Gβ 1 γ 2 (100 pmol) were incubated in 500 µL buffer for 16 h at 4℃ in the presence of AGS8 inhibitor. Five uL of mixtures were saved as “input”, then 20 µL of 50 % slurry of Glutathione Sepharose 4B was added. After 3 h incubation at 4°C, pellets were washed three times with 1000 µL buffer. This is a representative result from 4 independent experiments with similar results. The left panel and the right panels were captured from the same blot (see ). B, Effects of AGS8 inhibitor on VEGF-induced phosphorylation of VEGFR2, p38 MAPK and ERK1/2. Cell lysates (10–20 μg) from HUVECs treated with VEGF (10 ng/ml) for 10 min were subjected to immunoblotting, and signal intensity was quantified by densitometric analysis. In some groups, cells were treated with AGS8 inhibitor 30 min before stimulation. Values were normalized to total VEGFR2, p38 MAPK, and ERK1/2, respectively. Data shown are representative pictures from 5 independent experiments with similar results (n = 5). P < 0.05, control vs. VEGF (two-way ANOVA) in each of VEGFR2, p38 MAPK, and ERK1/2. ＊ P < 0.05 vs . VEGF without AGS8 inhibitor (one-way ANOVA with Dunnett’s multiple-comparison test).

Article Snippet: Antibodies against VEGFR2 (#2479), phospho-VEGFR2 (Tyr1175) (#3770), p38MAPK family proteins (#9212), phospho-p38 MAPK family proteins (Thr180/Tyr182) (#9211), ERK1/2 (p44/42 MAPK) (#9102), and phospho-ERK1/2 (p44/42 MAPK) (Thr 202/204) (#9101) were purchased from Cell Signaling Technology (Danvers, MA).

Techniques: GST Pulldown Assay, Incubation, Phospho-proteomics, Western Blot, Control, Comparison